A rapid method for determination of polar compounds in used frying fats and oils
نویسندگان
چکیده
La determinación de compuestos polares mediante cromatografía de adsorción es el método más aceptado en el análisis de aceites y grasas de fritura debido a su elevada exactitud y precisión. Sin embargo, la determinación es costosa, debido al elevado consumo de sílice y disolventes, y son necesarias varias horas para la obtención del resultado. En este estudio se propone una determinación alternativa rápida. Partiendo de miligramos de muestra disueltos en una solución de hexano con oleato de metilo como patrón interno, la fracción no polar, que contiene los triglicéridos no alterados y el patrón interno, es aislada mediante extracción en fase sólida. Posteriormente, la fracción no polar se analiza cuantitativamente en 15 min mediante cromatografía líquida de exclusión y la cantidad de fracción polar se determina por diferencia de peso. Se han calculado los factores de respuesta de triglicéridos y ésteres metílicos. El método se ha aplicado a seis muestras de aceites de girasol de distinto grado de insaturación evaluadas por triplicado y los resultados se han comparado con los obtenidos mediante la determinación gravimétrica basada en cromatografía en columna clásica de sílice. No se han encontrado diferencias significativas entre los dos grupos de resultados. Por otra parte, la repetibilidad fue excelente ya que los coeficientes de variación oscilaron entre 1.5 y 13% dependiendo del contenido en compuestos polares de los aceites.
منابع مشابه
International Union of Pure and Applied Chemistry Chemistry and the Environment Division Commission on Oils, Fats, and Derivatives*† Determination of Polar Compounds, Polymerized and Oxidized Triacylglycerols, and Diacylglycerols in Oils and Fats
A description is given of the development by collaborative study of two standardized methods for the determination of polar compounds in oils and fats by adsorption chromatography using silica minicolumns, and for quantification of polymerized triacylglycerols, oxidized triacylglycerols, and diacylglycerols in polar compounds by high-performance size-exclusion chromatography. The first procedur...
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